Fig. 2. (  A )Changes in glucocorticoid receptor (GR) protein (Western blot) level within the spinal cord dorsal horn after treatment (twice daily × 6 days) with either a vehicle or morphine (10 μg intrathecal) and one of the following GR oligodeoxynucleotides (5 μmol each, intrathecal): sense, antisense, or mixed base. Lane assignment: Veh = vehicle alone; O = an oligodeoxynucleotide alone; M/O = morphine plus an oligodeoxynucleotide. *  P < 0.05 as compared with the vehicle group. (  B )RU38486 (1 μg intrathecal) attenuated GR up-regulation within the spinal cord dorsal horn induced by morphine (10 μg intrathecal) administration. Both agents were given twice daily for 6 days. *  P < 0.05 as compared with the vehicle group. V = vehicle; M = morphine; R = RU38486. In  A and  B , RD refers to relative density (an average of triplicates) of each GR band as normalized against the corresponding loading band.