Fig. 3. Hypothermic preconditioning–induced acute phase of neuroprotection in rat cerebellar slices. Survival rate and morphologically intact rate of Purkinje cells are presented in A and B , respectively. C: Time control. I: oxygen-glucose deprivation (OGD) for 20 min at 37°C. 0HI, 1HI, 2HI, and 3HI: hypothermia (33°C for 20 min) applied immediately (0HI), 1 h (1HI), 2 h (2HI), and 3 h (3HI) before the OGD. 0HC, 1HC, 2HC, and 3HC: hypothermia applied at the corresponding time points to slices without being subjected to the OGD (see figure 1for the experimental protocols). *P < 0.01 compared with C group. N = 7 to 8 for each experimental condition. •= lowest and highest rates; between lines = 95% interval of the data; inside boxes = 25–75% interval, including the median of the data.