Functional imaging of the command interneurons AVA, AVD, AVE, and RIM, showing spontaneous activity at varying levels of anesthesia. Shown are example traces of individual animals are displayed (left), along with a histogram (right) of the normalized neuronal signals across all animals measured under that condition. The oppositional activity of AVB is shown in the unanesthetized state. (A) Control, unanesthetized (0% isoflurane). (B) At moderate levels of anesthesia (4% isoflurane). (C) At deep levels of anesthesia (8% isoflurane).