Nonconsecutive anesthesia exposures with sevoflurane induced cognitive impairment in young mice. The mice received anesthesia with 3% sevoflurane 2 h daily for nonconsecutive days (postnatal day [P] 6, P8, and P10) and were tested from P31 to P37 in the Morris water maze (MWM). (A) Two-way analysis of variance (ANOVA) with repeated-measurement analysis showed a significant interaction of treatment (control vs. sevoflurane) and time (P31 to P37) on escape latency of MWM. The mice that received the sevoflurane anesthesia had longer escape latency as compared with that in the mice in the control group at P34, P35, P36, and P37. (B) The sevoflurane anesthesia decreased the platform-crossing times as compared with the control conditions in the mice at P37. N = 10 in each group. DMSO = dimethyl sulfoxide.