Fig. 3.
Nutlin-3 attenuated the sevoflurane-induced reduction in postsynaptic density 95 protein (PSD-95) level in the hippocampus neurons, synaptosomes of mice, and hippocampus tissues of mice. (A) Anesthesia with 4.1% sevoflurane plus dimethyl sulfoxide (DMSO) for 4 h (lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in hippocampus neurons of mice. The treatment with sevoflurane plus Nutlin-3 (lane 4) did not reduce PSD-95 levels as compared to the treatment with control conditions plus Nutlin-3 (lane 3). (B) Quantification of the Western blot showed that sevoflurane plus DMSO (black bar) decreased PSD-95 levels as compared to control conditions plus DMSO (white bar). Sevoflurane plus Nutlin-3 (striped bar) did not decrease PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 5.202, P = 0.034, two-way analysis of variance [ANOVA]). (C) Anesthesia with 4.1% sevoflurane plus DMSO for 4 h (lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in hippocampus synaptosomes of mice. The treatment with sevoflurane plus Nutlin-3 (lane 4) did not reduce PSD-95 levels as compared to the treatment with control conditions plus Nutlin-3 (lane 3). (D) Quantification of the Western blot showed that sevoflurane plus DMSO (black bar) decreased PSD-95 levels as compared to control plus DMSO (white bar). Sevoflurane plus Nutlin-3 (striped bar) did not reduce PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 12.680, P = 0.002, two-way ANOVA). (E) Anesthesia with 3% sevoflurane 2 h daily for 3 days plus DMSO (postnatal day [P] 6, P7, and P8; lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in the hippocampus tissues of mice harvested at P8. The sevoflurane anesthesia plus Nutlin-3 (lane 4) did not significantly decrease PSD-95 levels as compared to the control conditions plus Nutlin-3 (lane 3). (F) Quantification of the Western blot showed that the sevoflurane anesthesia plus DMSO (black bar) decreased PSD-95 levels as compared to the control conditions plus DMSO (white bar). The sevoflurane anesthesia plus Nutlin-3 (striped bar) did not reduce PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 9.045, P = 0.007, two-way ANOVA). N = 6 in each group.

Nutlin-3 attenuated the sevoflurane-induced reduction in postsynaptic density 95 protein (PSD-95) level in the hippocampus neurons, synaptosomes of mice, and hippocampus tissues of mice. (A) Anesthesia with 4.1% sevoflurane plus dimethyl sulfoxide (DMSO) for 4 h (lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in hippocampus neurons of mice. The treatment with sevoflurane plus Nutlin-3 (lane 4) did not reduce PSD-95 levels as compared to the treatment with control conditions plus Nutlin-3 (lane 3). (B) Quantification of the Western blot showed that sevoflurane plus DMSO (black bar) decreased PSD-95 levels as compared to control conditions plus DMSO (white bar). Sevoflurane plus Nutlin-3 (striped bar) did not decrease PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 5.202, P = 0.034, two-way analysis of variance [ANOVA]). (C) Anesthesia with 4.1% sevoflurane plus DMSO for 4 h (lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in hippocampus synaptosomes of mice. The treatment with sevoflurane plus Nutlin-3 (lane 4) did not reduce PSD-95 levels as compared to the treatment with control conditions plus Nutlin-3 (lane 3). (D) Quantification of the Western blot showed that sevoflurane plus DMSO (black bar) decreased PSD-95 levels as compared to control plus DMSO (white bar). Sevoflurane plus Nutlin-3 (striped bar) did not reduce PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 12.680, P = 0.002, two-way ANOVA). (E) Anesthesia with 3% sevoflurane 2 h daily for 3 days plus DMSO (postnatal day [P] 6, P7, and P8; lane 2) decreased PSD-95 levels as compared to control conditions plus DMSO (lane 1) in the hippocampus tissues of mice harvested at P8. The sevoflurane anesthesia plus Nutlin-3 (lane 4) did not significantly decrease PSD-95 levels as compared to the control conditions plus Nutlin-3 (lane 3). (F) Quantification of the Western blot showed that the sevoflurane anesthesia plus DMSO (black bar) decreased PSD-95 levels as compared to the control conditions plus DMSO (white bar). The sevoflurane anesthesia plus Nutlin-3 (striped bar) did not reduce PSD-95 levels as compared to control conditions plus Nutlin-3 (gray bar; F = 9.045, P = 0.007, two-way ANOVA). N = 6 in each group.

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