Fig. 7.
Metabotropic glutamate receptor subtype 1 (mGluR1) modulates voltage-gated potassium channel subunit 2 (Kv2) current via protein kinase C (PKC). (A, top) Time course of changes in normalized total outward current amplitudes of chronic constriction injury (CCI) neurons induced by 10 μM Che. (A, bottom) Representative traces of current amplitude at +30 mV (left) and comparison of current density (right) show a prominent increase during Che application. One-way repeated-measures ANOVA and post hoc Bonferroni multiple comparison tests, n = 7; ** versus pre. (B, top) Time course of changes in normalized total outward current amplitudes of CCI neurons induced by 10 μM Che, in the presence of 100 nM guangxitoxin-1E (GxTX). (B, bottom) Application of GxTX dramatically decreases the total outward current density and subsequent additional application of Che exerts no effect. One-way repeated-measures ANOVA and post hoc Bonferroni multiple comparison tests, n = 6; ** versus pre. (C, top) Time course of changes in normalized total outward current amplitudes of CCI neurons induced by 10 μM 9-(dimethylamino)-3-(hexahydro-1H-azepin-1-yl)pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4(3H)-one (A841720), in the presence of 20 μM PMA. (C, bottom) Application of phorbol 12-myristate 13-acetate (PMA) significantly reduces the total outward current density. Subsequent application of A841720 shows no additional effect. One-way repeated-measures ANOVA and post hoc Bonferroni multiple comparison tests, n = 7; ** versus pre. (D, top) Time course of changes in normalized total outward current amplitudes of sham neurons induced by 15 μM (RS)-3,5-dihydroxyphenylglycine (DHPG), in the presence of 10 μM Che. (D, bottom) Application of Che has no effect on total outward current density. Subsequent application of DHPG also shows no additional effect. One-way repeated-measures ANOVA and post hoc Bonferroni multiple comparison tests, n = 6. (E and F) Neither the application of forskolin (E) nor H89 (F) rescues the reduced total outward current in CCI cholera toxin subunit B (CT-B)+ neurons. One-way repeated-measures ANOVA, all groups n = 7. *P < 0.05, **P < 0.01.