Fig. 9.
Vincristine (VCR)-induced allodynia, up-regulation of tumor necrosis factor-α (TNF-α), and calcitonin gene–related peptide (CGRP) C-fiber sprouting in the dorsal horn are abolished by intrathecal injection of nuclear factor-κB (NF-κB) inhibitor. (A) The time courses of paw withdrawal thresholds in vehicle (Veh), pyrrolidine dithiocarbamic acid (PDTC), VCR, and PDTC + VCR groups are shown. (B) The Western blots show the levels of TNF-α in different groups, and the histograms show the summary data. (C) The photographs show CGRP C-fibers in the dorsal horn of different groups (a–d), and the histograms show that the up-regulation of CGRP in both laminas I to II and laminas III to IV induced by VCR was blocked by NF-κB inhibitor PDTC (e–f). n = 6 per group. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the Veh group; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with the VCR group.