Fig. 3.
Immunofluorescent and immunoblot analysis of metastasis-related protein changes after sevoflurane exposure. Caski and HeLa cells were treated with or without 2% sevoflurane and assessed with immunofluorescent analysis of histone deacetylase 6 (HDAC6; A) 4 h after gas exposure. Time course experiments were evaluated with Western blot against HDAC6 (B) at different time points (0, 4, 6, 18 and 24 h) after gas exposure. The expression of HDAC6 protein in cervical cancer cells transfected with HDAC6 small interfering RNA was analyzed by Western blot analysis (C). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) level was used as internal controls. The data are presented as means ± SD (n = 5). Unpaired t test (immunofluorescence), one-way ANOVA with Dunnett test (Western blot), and one-way ANOVA with Newman–Keuls test (C) were used. *P < 0.001 versus control or as present. DAPI, 4,6-diamidino-2-phenylindole; H Si, HDAC6 small interfering RNA; Scr Si, scramble small interfering RNA.