Fig. 2.
Aβ-mediated biphasic eEPSP-eIPSP evoked in PKCγ-tdTomato neurons. (A) Schematic showing PKCγ-tdTomato+ neurons in lamina IIi that receive Aβ input and feed-forward inhibition driven by Aβ input. (B) A biphasic synaptic response was evoked by dorsal root stimulation at Aβ intensity. The inset shows the latency gap between the eEPSP and eIPSP when the membrane potential was held at -70 mV or 0 mV. (C) Strychnine blocks the dorsal root evoked IPSP and generates APs in PKCγ+ neurons at resting membrane potentials. Successive application of bicuculline does not change the amplitude of AP. (D) Application of bicuculline blocks eIPSP and strychnine generates long-lasting APs in PKCγ+ neurons at resting membrane potentials. (E) An example of a PKCγ-tdTomato neuron receiving mixed GABAergic and glycinergic inputs. (F) An example of a PKCγ-tdTomato neuron receiving pure glycinergic input. (G) An example of a PKCγ-tdTomato neuron receiving pure GABAergic input. (H) Pie chart analysis of numbers of PKCγ+ neurons receiving mixed afferent inputs, pure glycinergic, and pure GABAergic afferent inputs. AP, action potential; eEPSP, evoked monosynaptic excitatory postsynaptic potential; eIPSP, polysynaptic inhibitory postsynaptic potential; GABA, γ-aminobutyric acid; GABAergic, γ-aminobutyric acid–mediated; HP, holding potential; PKCγ, protein kinase C γ; PKCγ+, protein kinase C γ expressing.