Fig. 1.
Effects of dimethyl fumarate treatment on reflexive and operant nociceptive measures after spared nerve injury (SNI). (A) Beginning 14 days after SNI or sham surgery, dimethyl fumarate or vehicle was orally administered once per day for 7 days (days 1 and 2, 30 mg/kg; days 3 and 4, 100 mg/kg; days 5 to 7, 300 mg/kg). Mechanical allodynia was assessed using the von Frey test. SNI-induced allodynia was attenuated by dimethyl fumarate in a dose-dependent fashion. n = 4 rats/group. (B, C) Beginning 14 days after SNI or sham surgery, dimethyl fumarate or vehicle was orally administered for 5 days (300 mg/kg; once per day). (B) Von Frey thresholds for mechanical allodynia. n = 6 rats/group. (C) Latency to cross noxious probes and enter the dark compartment during the final 300-s mechanical conflict-avoidance test after 4 days of treatment. n = 5 in the naïve-dimethyl fumarate group, n = 6 rats in all other groups. Data are mean ± SD or individual data points; *P < 0.05, **P < 0.01, ***P < 0.001.