![Quantitative real-time polymerase chain reaction analysis of biologic markers associated with inflammation (tumor necrosis factor [TNF]-α) and muscle proteolysis (muscle RING-finger-1 [MuRF1]). Data are presented as a box plot. Lines denote the median, and boxes delimit the 25th and 75th percentiles of eight animals per group. Relative gene expression was calculated as a ratio of the average gene expression values compared with the reference gene 36B4 and expressed as fold change relative to nonventilated (NV). Mann–Whitney U test with Bonferroni correction for multiple comparisons (P < 0.0167, PCV_VTvs. PSV, PCV_ΔPLvs. PSV, and PCV_ΔPL_Tivs. PSV). PCV_ΔPL, pressure-controlled ventilation with dynamic ΔPL similar to that achieved by pressure-support ventilation; PCV_ΔPL_Ti, pressure-controlled ventilation with dynamic ΔPL and inspiratory time (Ti) similar to that achieved by pressure-support ventilation; PCV_VT, pressure-controlled ventilation with the same VT of pressure-support ventilation; PSV, pressure-support ventilation adjusted to VT = 6 ml/kg; VT, tidal volume.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/132/2/10.1097_aln.0000000000003060/2/17ff04.jpeg?Expires=1716595444&Signature=bTL~OK5grA0~APp3aKyKivm80l-MvO0BX3YmcFNdbvKqipbKyWkfq6sM-chZOFr7SrpgNPAa46sp25gAbZ6VHd3OIP6y7rJN6mmLgKgMsYixnKCmALFOdlw1zlzRCluM07FfFuZmsjTm-9gag-5W65pkQM6tL4qTpyE~sk-SNbZmAudakc6I7iKnOhhToGiceBjJHO3HvP7o8xNQpfdpr0nJpA-6eT2zZJ1oAD~Pkcdxkx8jyFMZOd7O1HGyW0Htl513rz9KcyE-QepvACQEjdSs5eaweumdTgcR7NbLOoXZ9WSKCmc2CUuIJ4HyQ09TjJzZWvsoRCD5Bim2fAM36w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Quantitative real-time polymerase chain reaction analysis of biologic markers associated with inflammation (tumor necrosis factor [TNF]-α) and muscle proteolysis (muscle RING-finger-1 [MuRF1]). Data are presented as a box plot. Lines denote the median, and boxes delimit the 25th and 75th percentiles of eight animals per group. Relative gene expression was calculated as a ratio of the average gene expression values compared with the reference gene 36B4 and expressed as fold change relative to nonventilated (NV). Mann–Whitney U test with Bonferroni correction for multiple comparisons (P < 0.0167, PCV_VTvs. PSV, PCV_ΔPLvs. PSV, and PCV_ΔPL_Tivs. PSV). PCV_ΔPL, pressure-controlled ventilation with dynamic ΔPL similar to that achieved by pressure-support ventilation; PCV_ΔPL_Ti, pressure-controlled ventilation with dynamic ΔPL and inspiratory time (Ti) similar to that achieved by pressure-support ventilation; PCV_VT, pressure-controlled ventilation with the same VT of pressure-support ventilation; PSV, pressure-support ventilation adjusted to VT = 6 ml/kg; VT, tidal volume.
