Fig. 3.
Effects of compounds 1 to 9 on sodium currents in human embryonic kidney 293-Nav1.7 cells. Compound was applied at the beginning of the second minute for 2 min (denoted as a horizontal line above the x-axis). Sodium currents were recorded at equal intervals (every 20 s, 40 recordings per compound per cell). Data were analyzed with a two-way repeated measures ANOVA, followed by a post hoc test of the last timepoint Bonferroni-corrected for nine pairwise comparisons (each compound was compared with control). The time × treatment interaction term was statistically significant (ANOVA F[351, 1,482] = 3.710; P < 0.0001). At the last measurement, statistically significant difference in relative current from the control group was observed for compound 9 (0.71; 95% CI, 0.47 to 0.95; P < 0.0001), compound 8 (0.36; 95% CI, 0.11 to 0.61; P = 0.001), compound 7 (0.35; 95% CI, 0.08 to 0.61; P = 0.003), and compound 5 (0.29; 95% CI, 0.02 to 0.55; P = 0.028). The other compounds were not significantly different from control (P = 0.768 for compound 6; P > 0.999 for compounds 1 to 4). Sample size n = 3 to 7 cells per group.