Fig. 5.
Regulation of Tau phosphorylation and cognitive function by Nuak1 inhibitor HTH-01-015 in neonatal mice of both sexes. (A) Amounts of Tau–phosphorylated serine (PS) 356 and Tau–PS202/phosphorylated threonine (PT) 205 in the cortex of neonatal mice of both sexes and female reference group mice. (B) Summary of Tau-PS356 (n = 6 mice/group). (C) Summary of Tau-PS202/PT205 (n = 6 mice/group). (D, E) Escape latency (D) and platform-crossing number (E) of Morris water maze in neonatal mice for baseline and sevoflurane anesthesia groups after pretreatment with vehicle (n = 10 mice/group). (F, G) Escape latency (F) and platform-crossing number (G) of Morris water maze in neonatal mice for baseline and sevoflurane anesthesia groups after pretreatment with HTH-01-015 (n = 10 mice/group). The data are presented as means ± SD. All data are quantified and expressed as arbitrary units or real numbers compared with baseline group (nonanesthetized mice). One-way ANOVA was used for (B) and (C). Two-way ANOVA with repeated measurement was used for (D) and (F). Mann–Whitney test was used for (E) and (G). *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.05.