Ice slurry injection leads to long-lasting and reversible functional loss. (A) Toe spread and (B) walking scores of hind limbs injected with slurry. Data are presented as median with interquartile range. n = 8 per group; *P < 0.05, **P < 0.001 compared with the baseline by Friedman test followed by Dunn’s multiple comparisons test. (C) The graph shows paw withdrawal frequency (PWF) in response to plantar stimulation with 15 g von Frey hair. n = 8 per group; *P < 0.05, **P < 0.001 compared with the baseline by Friedman test followed by Dunn’s multiple comparisons test. (D) Comparison of the effects of slurry and room temperature control solution on mechanical pain with 15 g von Frey hair in treated hind limbs. Paw withdrawal frequency values at each time point are normalized by dividing it by the value in the same animal at baseline and results are shown as percentage of the baseline value. n = 8 per group; *P < 0.05, **P < 0.001 compared with the room temperature solution control group by two-tailed Mann–Whitney U test. RT, room temperature.