Fig. 7.
Transient receptor potential canonical (TRPC) 3 protein expression levels in cardiomyocytes. (A, B) Representative immunostaining images of TRPC3 proteins in cells in sham (A) and modified transverse aortic constriction rats (B). (C, D) Fluorescence intensities along the yellow lines shown in A (C) and B (D). (E) Fluorescence intensities at both sides of the plasma membrane (level S1 and level S2) and the minimal cytoplasmic intensity (level C). (F) The ratio of S to C levels (S/C ratio) in sham (n = 15 cells) and aortic constriction (n = 19 cells) rats. Data were not normally distributed and are shown with median (25th and 75th percentile box), 10th, and 90th percentiles (whiskers). **P < 0.01. S = 1/2 (S1 + S2). A.U., arbitrary unit.