Fig. 4.
Regional transcriptomics differ according to regional aeration and systemic lipopolysaccharide exposure. (A) Hierarchical clustering shows distinct gene expression profiles according to lung aeration and lipopolysaccharide exposure. Gene expression values (log2-transformed intensities) are scaled and depicted in color code format. Red: upregulated; blue: downregulated. (B) Number of differentially expressed genes (absolute fold change greater than 1.5 and adjusted P value less than 0.05) in atelectatic and aerated tissues in lipopolysaccharide (–) or lipopolysaccharide (+) conditions versus control. Red: upregulated; blue: downregulated. Connections between bars show the number of common genes between lung regions. (C) Gene sets differentially enriched in combination of aeration state and lipopolysaccharide exposure versus control. Cells are colored according to normalized enrichment scores, and “X” denotes no significance (adjusted P value greater than 0.05). TGF, transforming growth factor.