Fig. 7.
Differentially expressed genes in the dorsal root ganglion of chronic constriction injury mice with or without PRDM1 knockdown determined by RNA sequencing technology. (A) Scatter plot showing the upregulated and downregulated genes in ipsilateral lumbar 4 and lumbar 5 dorsal root ganglions in the chronic constriction injury + short hairpin RNA group compared with the chronic constriction injury + Scram group. Red dots represent genes with statistically significant upregulation of expression, blue dots represent genes with statistically significant downregulation of expression, and gray dots represent genes with no significant difference. (B) Heat map showing hierarchical clustering of differentially expressed genes of mice in the chronic constriction injury + short hairpin RNA group compared with the chronic constriction injury + Scram group. Upregulated and downregulated genes are colored in red and green, respectively. (C through I) Seven differentially expressed genes identified by RNA sequencing (shown in table 1) and verified by quantitative polymerase chain. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 versus chronic constriction injury + Scram group by two-tailed unpaired t test, n = 6 per group. PRDM1, positive regulatory domain I–binding factor 1.