Fig. 4.
Isoflurane at 0.5% produces time-dependent hyperactivity and sedation in vivo. (A) Representative traces of a mouse in control (upper) and 0.5% isoflurane (middle two), as well as during recovery (lower). (B) Upper: Representative power spectrogram of ripple recording. Lower: The time-course of travel distance and mean speed under 0.5% isoflurane. Purple dots are travel distance, and blue dots are mean speed. (C) Time-course of travel distance and mean speed of control mice. Purple dots are travel distance, and blue dots are mean speed. The time-course of travel distance (D), mean speed (E), and percentage of resting time (F) of mice between control and 0.5% isoflurane. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by a two-way repeated-measures ANOVA followed by post hoc Bonferroni multiple comparison test (B to F; n = 14/group; 7/7 males/females).