Diaphragmatic protein oxidation in the control group (Con) and interventional group treated with 24 h of mechanical ventilation (MV) during pentobarbital exposure (P-CMV) or during dexmedetomidine exposure (D-CMV). (A) Representative immunoblot for protein oxidation (oxyblot) and β-tubulin in each group. (B) Oxidative index defined as the ratio between densitometric values of the oxidized proteins bands to β-tubulin. Values are displayed as means ± SD. All data presented as integrated density value (IDV).