Diaphragmatic protein kinase B (Akt)/forkhead box O–1 (FoXO1) measures in the control group (Con) and interventional group treated with 24 h of mechanical ventilation (MV) during pentobarbital exposure (P-CMV) or during dexmedetomidine exposure (D-CMV). (A) Representative immunoblots by group for phosphorylated Akt (p-Akt), total Akt, α-tubulin. (B) Levels of p-Akt and total Akt normalized to α-tubulin. (C) Representative immunoblots by group for phosphorylated FoXO1 (p-FoXO1), total FoXO1, and β-tubulin. (D) Levels of p-FoXO1 and total FoXO1 normalized by β-tubulin. Values are displayed as means ± SD. All data presented as integrated density value.