Fig. 2.
Photomicrographs showing extracellular signal-regulated kinase (ERK) phosphorylation in glial cells in the cuneate nucleus (CN) after chronic constriction injury (CCI). Double immunofluorescence labeling for phosphorylated ERK (p-ERK) (red; B, E, H), NeuN, a neuronal marker (green; A), glial fibrillary acidic protein (GFAP), an astrocyte marker (green; D), and OX-42, a microglia marker (green; G) in the ipsilateral CN at day 7 after CCI is shown. Merged images are displayed in the right panels (C, F, I). The results revealed that p-ERK was coexpressed with GFAP and OX-42, suggesting the expression of p-ERK in the astrocytes and microglia. Scale bar = 50 μm.