Fig. 2.
Transient receptor potential ankyrin 1 (TRPA1) activators reduce myocardial infarct size. (A) Chemical structure of TRPA1 activators. (B) Experimental protocol for myocardial ischemia–reperfusion studies. Rats were given a TRPA1 activator (ASP [ASP7663] or optovin) or vehicle (dimethyl sulfoxide [DMSO]) 5 min before 30 min of left anterior descending coronary artery ligation to cause ischemia followed by 2 h of reperfusion. (C) Infarct size per area at risk percentage for each experimental group. Data points represent individual biologic results for each experiment in addition to values presented as mean ± SD (n = 6); *P < 0.001 versus DMSO group. (D) Representative images of left ventricle area at risk. Infarcted areas are unstained and remain white, while viable tissue is stained red by triphenyl tetrazolium chloride.