Fig. 4.
Anesthesia-induced apoptotic cell death in 2-week-old dentate granule cells does not lead to alterations in neuronal proliferation in this population. Confocal maximum projections show green fluorescent protein (GFP) plus Ki67 (A) and GFP plus calretinin (B) immunostaining in the hippocampal granule cell layer. Double-labeled cells are denoted by arrows. Scale bar = 30 μm. Scatterplots show the percentage of GFP-expressing cells double-labeled with Ki67 (C) or calretinin (D). Circles represent individual animal means, while squares reflect group means ± SEM. The two groups were statistically equivalent for both Ki67 and calretinin.