Anesthesia-induced apoptotic cell death in 2-week-old dentate granule cells (DGCs) does not lead to long-term diminution in neuronal density in this population. Confocal maximum projections show immunostaining for green fluorescent protein (GFP) in representative 2-month-old animals exposed to 6 h of 1.5% isoflurane (anesthesia) or room air (control) on postnatal day 21. No differences between control and anesthesia-treated mice were found. Scale bar = 250 μm. Scatterplot shows the density of GFP-expressing cells in the dentate granule cell body layer. Circles represent individual animal means, while squares reflect group means ± SEM. The two groups were statistically equivalent (P = 0.915, Student’s t test).