The effects of single chemokine-C-motif ligand 1 (XCL1) neutralizing antibody (nAb) administrations on allodynia and hyperalgesia in streptozotocin (STZ; 200 mg/kg; intraperitoneal)-induced diabetic neuropathic pain in mice measured at day 7 after STZ injection. The effects of single intrathecal vehicle (V) or nAb XCL1 antibody administrations (500 ng, 1 μg, 2 μg, 4 μg, and 8 μg/5 μl) on developed mechanical allodynia (von Frey test; A) and thermal hyperalgesia (cold plate test; B) were measured at 1, 4, 24, and 96 h after nAb administration at day 7 after STZ injection. Data are presented as the means ± SD (6 to 13 mice per group). The results were evaluated using one-way ANOVA followed by Bonferroni test for comparisons of selected pairs; #P < 0.05, ##P < 0.01, and ###P < 0.001 compared the V- versus nAb-treated STZ-induced diabetic neuropathic pain in mice at respective time points.