Fig. 4.
Immunohistochemistry in the streptozotocin (STZ)-induced diabetic neuropathy in mice. Representative immunofluorescent images from colocalization analysis performed on spinal cord, paraffin-embedded 7-μM microtome slices from streptozotocin model: chemokine-C-motif receptor 1 (XCR1; red) with neuronal marker neuronal nucleus (NeuN; green; A–C), XCR1 (red) with microglia marker ionized calcium-binding adaptor molecule 1 (IBA1; green; D–F), and XCR1 (red) with astroglia marker glial fibrillary acidic protein (GFAP; G–I). Dorsal part of lumbar spinal cord (dissected at day 7 after STZ injection) was shown as a representative section on each of selected images. Scale bars: 25 µm.