Fig. 5.
Lumen-to-blood passage of a small molecule by a magnetic resonance imaging–based assay. (A) Schematic illustration of a contrast agent, gadodiamide (molecular weight = 574 Da), instilled into the ligated jejunal sac. (B) Representative magnetic resonance images of rat plasma samples and standard curve of known concentration of gadodiamide in plates. Left: Standard concentration of gadodiamide prepared in two-fold serial dilution. Right: Plasma samples from three rat groups. (C) Quantification of the plasma concentration of gadodiamide in rats after 240 min as an index of gut permeability. The error bars represent 2 SEM. Dex = dexmedetomidine; LPS = lipopolysaccharide.