Fig. 5.
Amphiregulin (Areg) expression in different phenotypes of both alveolar macrophages (AMs) and peritoneal macrophages (PMs). Mouse AMs and PMs were treated with phosphate-buffered saline (M0), 1 μg/ml of lipopolysaccharide (M1) or 20 ng/ml of interleukin-4 (M2) for 4 or 24 h. Areg mRNA expressions in AMs (A) and PMs (C) treated for 4 h were detected by quantitative reverse transcription polymerase chain reaction. Areg expression was normalized to β-actin expression levels, and the mean value for M0 was set to 1. Data were shown as mean ± SD of five independent samples. Areg protein concentrations in supernatants of AMs (B) and PMs (D) treated for 24 h were detected by bicinchonininc acid . Values are mean ± SD of five independent experiments. *** P < 0.001 versus M0; one-way ANOVA Bonferroni posttest.

Amphiregulin (Areg) expression in different phenotypes of both alveolar macrophages (AMs) and peritoneal macrophages (PMs). Mouse AMs and PMs were treated with phosphate-buffered saline (M0), 1 μg/ml of lipopolysaccharide (M1) or 20 ng/ml of interleukin-4 (M2) for 4 or 24 h. Areg mRNA expressions in AMs (A) and PMs (C) treated for 4 h were detected by quantitative reverse transcription polymerase chain reaction. Areg expression was normalized to β-actin expression levels, and the mean value for M0 was set to 1. Data were shown as mean ± SD of five independent samples. Areg protein concentrations in supernatants of AMs (B) and PMs (D) treated for 24 h were detected by bicinchonininc acid . Values are mean ± SD of five independent experiments. *** P < 0.001 versus M0; one-way ANOVA Bonferroni posttest.

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