Fig. 4.
Increased phosphorylation of NF-κB p65 is induced in the CX3CL1-positive neurons after paclitaxel (Pacl) treatment. (A) The expression of phosphorylated p65 at Ser311 was significantly increased after paclitaxel treatment. n = 6 in each group; **P < 0.01 vs. corresponding control group (Con). (B) Increased phosphorylation of p65 was significantly induced after paclitaxel treatment compared with control group. Scale bar, 200 µm. (C) The immunofluorescence staining of p-p65 (red) was colocalized with NeuN (neuron marker, green) and OX-42 (microglia marker, green) but not with glial fibrillary acidic protein (GFAP) (astrocyte marker, green). Scale bar, 50 µm. (D) Double immunofluorescence staining indicated colocalization of p-p65 with CX3CL1 in the spinal neurons. Scale bar, 50 µm.