Fig. 2.
Different behavioral endpoints reveal syntaxin1A-mediated resistance and hypersensitivity in adult Drosophila. (A) Baseline locomotion endpoint from the startle-induced locomotion assay showing ΔEC50 ± standard error of the estimate (isoflurane vol% atm) for the baseline endpoint in syxH3-C (black) and syxKARRAA (gray) in a wild-type background (isoCJ1). ΔEC50 is calculated by subtracting the genetic background EC50 (isoCJ1) from the experimental syntaxin1A strain (n = 40 flies per genotype). syxKARRAA genetic control is syxWT in the isoCJ1 background. **P < 0.01, ***P < 0.001, calculated by extra sum-of-squares F test between estimated EC50. (B) Schematic of the fly coordination assay is shown (flies are not shown to scale). The tubes are commercially available glass tubes, 100 ml volume. The tubes were sealed with a rubber stopper, and the side arm of the tube was sealed with Teflon tape, and a small cap with septa which would facilitate the insertion of a syringe. After anesthetic injection, the number of flies in the bottom of the tube is counted every 10 s. Data shown are the mean time for 50% of the flies to become anesthetized, error bars represent the SEM; n >10 experiments per genotype. (C) The mean time for 50% of syxH3-C (black) and syxKARRAA (gray) to become anesthetized to 3 μl isoflurane in a wild-type background (isoCJ1) is shown, subtracting the experimental syntaxin1A strain from the time taken for 50% of the genetic control (isoCJ1) to become anesthetized. *P< 0.05, t test comparing means. (D) The mean time for 50% of syxH3-C (black) and syxKARRAA (gray) to become anesthetized to 2 μl halothane in a wild-type background (isoCJ1) is shown, subtracting the experimental syntaxin1A strain from the time taken for 50% of the genetic control (isoCJ1) to become anesthetized. *P < 0.05, nonparametric t test comparing means.