Fig. 7.
Mitochondrial gene expression in polymicrobial sepsis. WT and TLR2-/- mice were subjected to Sham or CLP procedure. After 24 h of surgery, liver, heart, and peritoneal cells were harvested. Total RNA was extracted and mitochondrial gene expression was measured by quantitative reverse transcription polymerase chain reaction and normalized to GAPDH levels. *P < 0.05, **P < 0.01, ***P < 0.001. The numbers of animals in each group: A, n = 6 mice in each group; B, n = 6 in each group; C, Tfam, n = 11 in WT-sham, n = 9 in TLR2KO sham, n = 6 in CLP groups; COX-2: n = 9 in WT-sham, n = 9 in TLR2KO sham, n = 5 in WT CLP group, n = 6 in TLR2KO CLP group. Each error bar represents mean ± SEM. CLP = cecum ligation and puncture; COX-2 = cytochrome c oxidase subunit II; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; Tfam = mitochondrial transcript factor A; TLR2KO = TLR2 knockout; WT= wild type.

Mitochondrial gene expression in polymicrobial sepsis. WT and TLR2-/- mice were subjected to Sham or CLP procedure. After 24 h of surgery, liver, heart, and peritoneal cells were harvested. Total RNA was extracted and mitochondrial gene expression was measured by quantitative reverse transcription polymerase chain reaction and normalized to GAPDH levels. *P < 0.05, **P < 0.01, ***P < 0.001. The numbers of animals in each group: A, n = 6 mice in each group; B, n = 6 in each group; C, Tfam, n = 11 in WT-sham, n = 9 in TLR2KO sham, n = 6 in CLP groups; COX-2: n = 9 in WT-sham, n = 9 in TLR2KO sham, n = 5 in WT CLP group, n = 6 in TLR2KO CLP group. Each error bar represents mean ± SEM. CLP = cecum ligation and puncture; COX-2 = cytochrome c oxidase subunit II; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; Tfam = mitochondrial transcript factor A; TLR2KO = TLR2 knockout; WT= wild type.

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