Increased ion calcium-binding adaptor molecule-1 (Ibal) expression in microglia of the spinal cord dorsal horn is unaltered by the administration of AM251. Activity of microglia with and without AM251 in spinal dorsal horn at level of lumbar 1–2 was studied at day 7 after burn using specific fluorescent tag, Iba1. Groups studied included sham burn animals treated with vehicle (Sham + Veh), ipsilateral side of burn treated with vehicle (Burn + Veh), contralateral side to burn treated with vehicle (Contra + Veh), burn injured side treated with AM251 (Burn + AM251), and contralateral side to burn treated with AM251 (Contra + AM251). Scale bars for A and B are 100 μm and for Ca, Cb, Da, and Db are 25 μm. Burn injury, relative to contralateral side, activates microglia on the ipsilateral side. Treatment with AM251 did not alter the activation of microglia on the ipsilateral side of burned mice compared with ipsilateral side treated with vehicle (n = 3 per group).