Fig. 5.
The effect of isoflurane (Iso) exposure and rapamycin treatment on myelination in fimbria. (A) Myelination was quantitatively analyzed with myelin basic protein immunostaining. The myelin basic protein (MBP) intensity in isoflurane-exposed mice was reduced compared to control conditions and is increased with rapamycin treatment. For this analysis, the same exact photo setting was performed for all groups. Asterisk in photos: CA3 of hippocampus; fi: fimbria of hippocampus; LV: lateral ventricle. Scale bar = 200 μm. (B) Western blot data indicated expression of myelin basic protein was decreased by isoflurane exposure and then elevated by rapamycin treatment. (C) Electron microscopy analysis was performed in fimbria parasagittal ultrathin sections. The ratio of axonal caliber over diameter of myelinated fiber (g-ratio) was increased in isoflurane-exposed animals (it means decreased myelin thickness) compared to control, and rapamycin treatment reversed this change. Scale bar = 0.5 μm. n = 8 for each group in A and B, and n=100 for each group in C. One-way ANOVA; *P < 0.05; **P < 0.01. Error bars: SD.