Fig. 6.
Histone accumulation in the organs and plasma levels of high morbidity group box-1 after intestinal ischemia–reperfusion. (A) Intense histone accumulation was exhibited in the jejunum and liver harvested from mice subjected to 30-min ischemia followed by 3 h of reperfusion. Changes in the histone level in each organ were calculated by subtracting the average histone contents in sham-operated mice from histone measurements in the intestinal ischemia–reperfusion (IR)-injured mice (intestine vs. liver, P = 0.011; intestine vs. lung, P = 0.003; intestine vs. kidney, P = 0.011; liver vs. lung, P = 0.008; liver vs. kidney, P = 0.011; lung vs. kidney, P = 0.241; n = 7 mice per group, n = 28 total). Histone measurements in the sham-operated mice are also shown (n = 7 mice per group, n = 28 total). (B to D) The impact of 10 mg/kg recombinant thrombomodulin (rTM) on histone levels in the liver (B; sham vs. IR group, P = 0.015; IR vs. IR + rTM group, P = 0.030; n = 7 mice per group, n = 21 total), jejunum (C; sham vs. IR group, P = 0.025; IR vs. IR + rTM group, P = 0.752; n = 7 mice per group, n = 21 total), and plasma after intestinal IR (D; sham vs. IR group, P = 0.001; IR vs. IR + rTM group, P = 0.024; n = 7 mice per group, n = 21 total). (E) Levels of high morbidity group box-1 (HMGB1) in the plasma (sham vs. IR group, P = 0.002; IR vs. IR + rTM group, P = 0.025; n = 7 mice per group, n = 21 total). The histone and HMGB1 levels in the plasma are exhibited as whisker box plots wherein the middle line indicates the median; the upper and lower box lines indicate the third and first quartiles, respectively; and the whiskers extend to the highest and lowest values within 1.5 times the interquartile range from the box edges. Outliers beyond the whiskers are indicated as dots. #P < 0.05 versus all other groups; *P < 0.05 versus respective control. AU, absorbance unit.