Fig. 7.
Isoflurane has no effect on postsynaptic density protein-95, Drosophila disc large tumor suppressor, and zonula occludens-1 (PDZ) domain–mediated interactions between γ-aminobutyric acid type B (GABAB) receptor subunit 2 and PAPIN or Mupp1 in an yeast two-hybrid system. (A) No concentration of isoflurane tested inhibited the growth of yeast cells harboring pGADT7-GABABR2 and pGBKT7-PAPIN PDZ1 or pGBKT7-Mupp1 PDZ13. Consistent with our previous study,15 isoflurane dose-dependently inhibited the growth of yeast cells harboring pGADT7-PSD-95 PDZ2 and pGBKT7-NR2B C-terminus, which were used as a positive control. (B) Yeast growth is shown as a percent of control, relative to isoflurane concentration (n = 6). *P < 0.05 and **P < 0.01 versus 0.00 mM isoflurane. GABABR2 = GABAB receptor subunit 2; PSD-95 = postsynaptic density protein-95.