Fig. 3.
Halothane and isoflurane dose-dependently disrupt postsynaptic density protein-95, Drosophila disc large tumor suppressor, and zonula occludens-1 (PDZ) domain–mediated interactions between AMPA receptor subunit GluR2 and glutamate receptor–interacting protein (GRIP) or protein interacting with c kinase 1 (PICK1) in an yeast two-hybrid system. (A−C) The effect of halothane on the growth of yeast cells harboring pGADT7-GluR2 CT and pGBKT7-GRIP PDZ4,5 (n = 3). (A) Halothane dose-dependently inhibited the yeast growth. (B) Yeast growth is shown as a percent of control, relative to halothane concentration. *P < 0.05 versus 0.00 mM halothane. (C) Binned scatter plots of A. (D−F) The effect of isoflurane on the growth of yeast cells harboring pGADT7-GluR2 CT and pGBKT7-PICK1 PDZ (n = 3). (D) Isoflurane dose-dependently inhibited the yeast growth. (E) Yeast growth is shown as a percent of control, relative to isoflurane concentration. *P < 0.05 versus 0.00 mM isoflurane. (F) Binned scatter plots of D. CT = C-terminus.