Fig. 1. Experimental protocols for in vivo  studies. Mice were subjected to pretreatment with alloxan and/or the anesthetic preconditioning (APC) protocol (30 min isoflurane) followed by left anterior descending coronary artery occlusion for 30 min and 2 h reperfusion (n = 8 per group) (A) . Experimental protocols for in vitro  studies. Cardiac myocytes were isolated and pretreated according to the protocol before exposure to simulated ischemia/reperfusion (n = 6 per group) (B) .