Fig. 4.  The responses of acid-sensing ion channel 3 (ASIC3) knockout (KO; n = 20) and wild-type (WT; n = 19) mice did not differ after spinal nerve ligation (SNL). Data are shown as mean ± SE in all figures. (A ) SNL resulted in significant heat hyperalgesia in both ASIC3 KO and WT mice. The average withdrawal latency to a radiant heat stimulus is plotted as a function of time after the lesion. **P < 0.01; ***P < 0.001 ipsilateral (ipsi) versus  contralateral (contra) paw. (B ) SNL resulted in significant and comparable hyperalgesia to punctate mechanical stimuli in ASIC3 KO and WT mice. *P < 0.05; **P < 0.01; ***P < 0.001 ipsilateral versus  contralateral paw in WT animals; # P < 0.05; ## P < 0.01; ### P < 0.001 ipsilateral versus  contralateral paw in KO mice. (C ) Detailed representation of the response frequency to different von Frey forces 14 days after the SNL (marked with a rectangle in B ). WT and KO animals did not significantly differ in their sensitivity to any force tested. See also the figure in Supplemental Digital Content 2, https://links.lww.com/ALN/A609. (D ) SNL resulted in significantly enhanced responses to the mechanical stimulus of stroking in both the ASIC3 KO and WT mice. The average percentage of trials in which the animal responded to stroking of the skin with a von Frey filament is plotted as a function of time after the lesion. Although ASIC3 KO animals were more responsive than WT mice to stroking stimulation at baseline, the level of hyperalgesia that developed after nerve injury was comparable in the two genotypes. ***P < 0.001 significant effect of time by Friedman analysis of variance. # P < 0.05 knockout versus  WT mice.