Fig. 2. (A ) Coadministration of yohimbine (3.0 + Y) at a subcutaneous dose of 1 mg/kg reversed the antihyperalgesic effect induced by MPV-2426 at a dose of 3 μg. Yohimbine alone (Y) had no effect. (B ) Dose-dependence of the antihyperalgesic effect induced by MPV-2426 at time point 90 min postinjection. (C ) Dose-dependence of the antihyperalgesic effect induced by dexmedetomidine at time point 15 min postinjection. (D ) Dose-dependence of the antihyperalgesic effect induced by clonidine at time point 90 min postinjection. (E ) The effect of preoperative treatment with intrathecally administered MPV-2426 (10 μg) and saline on the development of postoperative hyperalgesia at time point 24 h after the skin incision. The preoperative drug treatment was performed 60 min before surgery. (F ) The effect of intrathecal MPV-2426 (10 μg) on motor behavior in healthy control rats as assessed in the Rotarod test. The horizontal line indicates the cutoff latency (60 s). In graphs A–E  the median values are shown. Boxes extend from the twenty-fifth percentile to the seventy-fifth percentile and the whiskers from the smallest to the largest value. In graph F, the mean latencies and SD are shown. In A–E ; *P < 0.05; **P < 0.01 (Dunn test; reference: the saline-group = 0).