Fig. 3. Effect of propofol on vasopressin-induced accumulation of HSP27 in A10 cells. Cultured cells were pretreated with various doses of propofol for 20 min, then exposed to 0.1 μm vasopressin (closed circles) or vehicle (open circles) for 48 h. The concentrations of HSP27 in cell extracts were determined by a specific immunoassay. Each value represents the mean ± SD of triplicate determinations. Similar results were obtained with two other cell preparations.