Fig. 9. Thiopental induces mitochondrial apoptosis in Jurkat cells. Jurkats (1 × 106/ml) were stained with rhodamine 123 after incubation with increasing concentrations of thiopental (5–500 μg/ml) for 24 h ( black bars ) or 48 h ( gray bars ). Apoptotic cells are represented by a shift of mean fluorescence to the left. Data are given as percent of shifted cells from all cells measured. Results represent the mean ± SD of five separate experiments. Analysis of variance (Tukey test): * P < 0.05 with versus without thiopental.
