Fig. 8. Inhibition of thiopental and anti-CD95-induced caspase-3 activation by z-VAD-fmk. Jurkat cells (1 × 106/ml) were incubated with thiopental (250 μg/ml) and/or anti-CD95 antibody (CH11; 100 ng/ml) for 24 h after pretreatment with ( gray bars ) or without ( black bars ) the pan-caspase inhibitor z-VAD-fmk (20 μm). Caspase-3 activity was measured by DEVD-afc cleavage. Results represent the mean ± SD of five separate experiments. Student t test: * P < 0.05 with versus without thiopental; § P < 0.05 with versus without anti-CD95; # P < 0.05 with versus without z-VAD-fmk.
