Fig. 3. Thiopental is independent of CD95-induced apoptosis. Jurkat cells (1 × 10⁶/ml) were preincubated with (  shaded bars ) or without (  white bars ) an inhibitory anti-CD95 antibody (SM1/23; 500 ng/ml) 1 h before stimulation with thiopental (250 μg/ml) and/or an agonistic anti-CD95 antibody (CH11; 100 ng/ml) for 24 h. Data are reported as the percentage of apoptotic (Annexin-V–positive, propidium iodine–negative) cells (  A ) or secondary necrotic (Annexin-V–positive, propidium iodine–positive) cells (  B ). Results represent the mean ± SD of five separate experiments. Student  t test: *  P < 0.05 with  versus without thiopental; †  P < 0.05 with  versus without anti-CD95; #  P < 0.05 with  versus without SM1/23.