Fig. 2. Thiopental induces apoptosis and secondary necrosis of Jurkat cells. Cells (1 × 106/ml) were stained with fluorescein isothiocyanate–Annexin-V and propidium iodine after incubation with increasing concentrations of thiopental (5–500 μg/ml) for 24 h ( black bars ) or 48 h ( gray bars ). Data are reported as the percentage of apoptotic (Annexin-V–positive, propidium iodine–negative) cells ( A ) or secondary necrotic (Annexin-V–positive, propidium iodine–positive) cells ( B ). Results represent the mean ± SD of eight separate experiments. Analysis of variance (Tukey test): * P < 0.05 with versus without thiopental.