Fig. 4. The effect of thiopental on IκB-kinase (IKK) activity in CD3+cells. IKKα complexes were immunoprecipitated from cells that had been treated with tumor necrosis factor (TNF) α, thiopental, or the respective volumes of ppH2O as vehicle control, using an antibody against IKKα to perform an in vitro kinase assay. (Top ) Autoradiograph of a typical experiment. (Bottom ) Results of the quantitative densitometric analysis of all individual experiments. Data represent the median and 25–75% and 95% confidence intervals of six independent experiments. *P < 0.05 compared with TNF-α alone.