Fig. 5. Western blot analysis results for brain caspase-8 active fragments (10 kDa) and caspase-3 active fragments (17–20 kDa) in control, cardiopulmonary bypass (CPB), and deep hypothermic circulatory arrest (DHCA) groups: control (n = 8), CPB (n = 3), 1-h DHCA (n = 5), 4-h DHCA (n = 6), 8-h DHCA (n = 5), 24-h DHCA (n = 5), 72-h DHCA (n = 4), and 1-week DHCA (n = 3). Optical density relative to background is shown for each group, with corresponding images of bands below. Significant caspase-8 active fragments were found at 8 h and 24 h after DHCA and in the CPB group. Significant caspase-3 active fragments were found at 4 h, 24 h, and 72 h after DHCA (*P < 0.05).