Fig. 6. Isoflurane alone does not activate IKATP. KATPcurrent was monitored every 15 s during a test pulse to 0 mV from a holding potential of −40 mV. Current amplitude was measured at the end of the 100-ms test pulse. Control recordings were obtained for 25 min to allow for the diffusional exchange of 1 mm adenosine triphosphate from the pipette solution to the cell's interior prior to application of 1.0 mm isoflurane. Bimakalim (20 μm, Bim) was applied in the absence of isoflurane.