Fig. 1. Activation of IKATPby 2,4-dinitrophenol (DNP). (Top ) Sample whole cell current traces recorded in control, in the presence of 120 μm DNP, and in the presence of DNP + glibenclamide (Glib, 200 nm). Current was monitored at test potentials of −110, −30, and +10 mV from a holding potential of −40 mV. Current at −110 mV is the inward-rectifier K current. The outward current sensitive to glibenclamide is IKATP. (Bottom ) The corresponding current–voltage relation. Current amplitude was measured at the end of the 100-ms test pulses.