Fig. 5. Effects of benzodiazepines on the cyclic adenosine 3′,5′-monophosphate (cyclic AMP) efflux. (A ) Effects of corticotropin-releasing hormone (CRH) and forskolin. Cells were stimulated with 1 μm CRH or 10 μm forskolin. Cyclic AMP efflux is expressed as means ± SD from four experiments. *P < 0.05 versus nonstimulated control. (B ) Benzodiazepines dose-dependently increase cyclic AMP efflux induced by CRH or forskolin. In the presence of diazepam or midazolam, cells were stimulated with 1 μm CRH or 10 μm forskolin. The ratios of cyclic AMP efflux to that of nonstimulated controls are expressed as means ± SD from four experiments. *P < 0.05 versus cells stimulated with CRH without benzodiazepines. #P < 0.05 versus cells stimulated with forskolin without benzodiazepines. §P < 0.05 versus nonstimulated controls.