Fig. 6. Effects of genistein and isoflurane on single K^ATPchannel activity in an inside-out patch. Shown are 10-s recordings of K^ATPchannel activity at patch potential of +40 mV (left ) and corresponding all-points amplitude histograms (right ). Upward deflections indicates outward current. (C−) indicates closed state. (A ) At 0.2 mm ATP on the cytosolic side of the patch, genistein activated single channel current and increased channel open probability (Po). Coapplied with genistein, isoflurane failed to further increase Po. (B ) Summary of genistein effects shows a significant increase in Po by 50 μm genistein and no additional effect by isoflurane. *P < 0.05 versus  control, n = 10 patches. (C ) Summary of daidzein effects on Po. During experimental conditions as above, daidzein (50 μm, n = 3 patches) had no effect on Po, with or without isoflurane present.